The transcriptional coactivator MAML1 regulates p300 autoacetylation and HAT activity
نویسندگان
چکیده
MAML1 is a transcriptional coregulator originally identified as a Notch coactivator. MAML1 is also reported to interact with other coregulator proteins, such as CDK8 and p300, to modulate the activity of Notch. We, and others, previously showed that MAML1 recruits p300 to Notch-regulated genes through direct interactions with the DNA-CSL-Notch complex and p300. MAML1 interacts with the C/H3 domain of p300, and the p300-MAML1 complex specifically acetylates lysines of histone H3 and H4 tails in chromatin in vitro. In this report, we show that MAML1 potentiates p300 autoacetylation and p300 transcriptional activation. MAML1 directly enhances p300 HAT activity, and this coincides with the translocation of MAML1, p300 and acetylated histones to nuclear bodies.
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Role of Posttranslational Modifications in Regulation of Notch and Transcriptional Coactivator Mastermind-like 1
The Notch signaling is an evolutionary conserved pathway enabling short range cell-cell interactions, crucial for diverse developmental and physiological processes during embryonic and adult life. The Mastermind-like (MAML) family of transcriptional coactivator proteins has been shown to play an essential role in regulation of Notch-mediated transcription. Formation of DNA-bound ternary CSLNotc...
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